Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor - CONFREMO - Conférence des Recteurs et Présidents de la Région du Moyen-Orient
Article Dans Une Revue Micromachines Année : 2024

Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor

Résumé

Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5–20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R2 values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) yielded excellent results. This novel ePAD, fabricated using inkjet printing technology on paper, simplifies the process, reduces environmental impact, and lowers fabrication costs.
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hal-04738509 , version 1 (17-10-2024)

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Miguel Zea, Hamdi Ben Halima, Rosa Villa, Imad Abrao Nemeir, Nadia Zine, et al.. Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor. Micromachines, 2024, 15 (10), pp.1252. ⟨10.3390/mi15101252⟩. ⟨hal-04738509⟩
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